Td. Bradshaw et al., 2-(4-AMINOPHENYL)BENZOTHIAZOLES - NOVEL AGENTS WITH SELECTIVE PROFILES OF IN-VITRO ANTITUMOR-ACTIVITY, British Journal of Cancer, 77(5), 1998, pp. 745-752
2-(4-Aminophenyl)benzothiazole (CJM 126) elicits biphasic growth-inhib
itory effects against a panel of oestrogen receptor-positive (ER+) and
oestrogen receptor-negative (ER-) human mammary carcinoma cell lines
in vitro, yielding IC50 values in the nan range. Substitutions adjacen
t to the amino group in the 2-phenyl ring with a halogen atom or methy
l group enhance potency in sensitive breast lines (pM IC50 values). Tr
ansient biphasic dose responses were induced but rapidly eradicated af
ter specific drug exposure periods. Two human prostate carcinoma cell
lines were refractory to the growth-inhibitory properties of 2-(4-amin
ophenyl)benzothiazoles; IC50 values > 30 mu M were obtained. Potency a
nd selectivity were confirmed when compounds were examined in the Nati
onal Cancer Institute's Developmental Therapeutics screen; the spectru
m of activity included specific ovarian, renal, colon al; well as brea
st carcinoma cell lines. Moreover, comparing 6-day and 48-h incubation
s, the exposure time-dependent nature of the biphasic: response was co
rroborated. Differential perturbation of cell cycle distribution follo
wed treatment of MCF-7 and MDA 468 cells with substituted 2-(4-aminoph
enyl)benzothiazoles. In MDA 468 populations only, accumulation of even
ts in G(2)/M phase was observed. Two MCF-7 cell lines were established
with acquired resistance to CJM 126 (IC50 values > 20 mu M), which ex
hibit cross-resistance to substituted benzothiazoles, but equal sensit
ivity to tamoxifen and doxorubicin. Compared with standard anti-tumour
agents evaluated in the National Cancer Institute in vitro cell panel
, benzothiazoles revealed unique profiles of growth inhibition, sugges
ting a models) of action shared with no known clinically active class
of chemotherapeutic agents.