M. Sotoudeh et al., A STRAIN DEVICE IMPOSING DYNAMIC AND UNIFORM EQUI-BIAXIAL STRAIN TO CULTURED-CELLS, Annals of biomedical engineering, 26(2), 1998, pp. 181-189
The objective of this study is to design a new apparatus to allow the
control of the magnitude and frequency of dynamic stretch applied unif
ormly to cells cultured on a silicon elastic membrane. The apparatus i
s designed to produce equibiaxial dynamic stretches with area changes
ranging from 0% to 55% and frequencies ranging from 0 to 2 Hz. Homogen
eous finite strain analysis using triangles of markers was performed t
o compute the symmetric two-dimensional Lagrangian strain tensor on th
e membrane. Measurements of strain in both static and dynamic conditio
ns showed that the shear component of the strain tensor (E-rc) was nea
r zero, and that there was no significant difference between radial (E
-rr) and circumferential (E-cc) components, indicating the attainment
of equi-biaxial strain. Bovine aortic endothelial cells were transient
ly transfected with a chimeric construct in which the luciferase repor
ter is driven by TPA-responsive elements (TRE). The transfected cells
cultured on the membrane were stretched. The luciferase activity incre
ased significantly only when the cells were stretched by 15% or more i
n area. Cells in different locations of the membrane showed similar in
duction of luciferase activities, confirming that strain is uniform an
d equibiaxial across the membrane. (C) 1998 Biomedical Engineering Soc
iety.