AN IMPROVED COLONY-PCR METHOD FOR FILAMENTOUS FUNGI FOR AMPLIFICATIONOF PCR-FRAGMENTS OF SEVERAL KILOBASES

Authors
VANZEIJL CMJ VANDEKAMP EHM PUNT PJ SELTEN GCM HAUER B VANGORCOM RFM VANDENHONDEL CAMJJ
Citation
Cmj. Vanzeijl et al., AN IMPROVED COLONY-PCR METHOD FOR FILAMENTOUS FUNGI FOR AMPLIFICATIONOF PCR-FRAGMENTS OF SEVERAL KILOBASES, Journal of biotechnology, 59(3), 1998, pp. 221-224
Citations number
7
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Journal of biotechnology → ACNP
ISSN journal
01681656
Volume
59
Issue
3
Year of publication
1998
Pages
221 - 224
Database
ISI
SICI code
0168-1656(1998)59:3<221:AICMFF>2.0.ZU;2-#
Abstract
A method was developed to perform PCR directly on mycelial pellets or colonies treated with NOVOzym 234. The method allows rapid screening o f large numbers of transformants of both sporulating and non-sporulati ng fungi for the presence of (co)transforming plasmid copies or for sp ecific genetic modifications such as gene disruption and site specific integration. PCR fragments of at least 3.2 kb can be obtained. Using this method the identification of specific disruption mutants from Asp ergillus niger and Beauveria bassiana was carried out. (C) 1998 Elsevi er Science B.V.