DEVELOPMENT OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY USING RECOMBINANTCHICKEN ANEMIA VIRUS PROTEINS EXPRESSED IN A BACULOVIRUS VECTOR SYSTEM

Recombinant baculoviruses were constructed to express the putative pro teins VP1, VP2 or VP3 of the chicken anemia virus (CAV). The recombina nt VP1, VP2 or VP3 were detected by SDS-PAGE, and their molecular weig hts were 50, 30/27 and 16 kDa, respectively. The VP2 and VP3 reacted w ith sera from CAV-infected chickens in Western blot analysis and when used as an enzyme-linked immunosorbent assay (ELISA) antigen, but VP1 did not. Antibodies to CAV were detected, by ELISA using crude insect cell lysates containing VP2 or VP3, from 2 to 20 weeks or 2 to 7 weeks after CAV infection, respectively. These findings indicate that recom binant VP2 and VP3 expressed in the baculovirus vector system can be u sed as antigens to detect anti-CAV antibodies in ELISA.