Y. Gong et al., THE REPLICATIVE INTERMEDIATE MOLECULE OF BOVINE VIRAL DIARRHEA VIRUS CONTAINS MULTIPLE NASCENT STRANDS, Archives of virology, 143(2), 1998, pp. 399-404
The replication of bovine viral diarrhoea virus (BVDV) RNA is consider
ed to involve replicative intermediates (RI) and replicative forms (RF
) of virus RNA. These RNA species were radiolabelled metabolically by
H-3-uridine in BVDV-infected cells and separated by sucrose gradient c
entrifugation. RNA in the fractions was then digested with RNase A in
high salt (2 x SSC) and the RNase-resistance was determined. Using the
Baltimore formula, this led to the calculation that the RI of BVDV co
ntained 6-7 nascent strands on the template. This suggests that the st
ructure of the BVDV RI is similar to that of poliovirus and dengue 2 v
irus.