LARGE CAG CTG REPEAT TEMPLATES PRODUCED BY PCR, USEFULNESS FOR THE DIRECT METHOD OF CLONING GENES WITH CAG/CTG REPEAT EXPANSIONS/

Authors
PUJANA MA VOLPINI V ESTIVILL X
Citation
Ma. Pujana et al., LARGE CAG CTG REPEAT TEMPLATES PRODUCED BY PCR, USEFULNESS FOR THE DIRECT METHOD OF CLONING GENES WITH CAG/CTG REPEAT EXPANSIONS/, Nucleic acids research, 26(5), 1998, pp. 1352-1353
Citations number
21
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
26
Issue
5
Year of publication
1998
Pages
1352 - 1353
Database
ISI
SICI code
0305-1048(1998)26:5<1352:LCCRTP>2.0.ZU;2-0
Abstract
We report here a simple method for generating large CAG/CTG repeat seq uences, We have applied this method to clone the genomic sequence cont aining the CAG/CTG repeat and its upstream intronic sequence present i n spinocerebellar ataxia type 3 or Machado-Joseph disease (SCA3/MJD) b y a modified DIRECT method, With these modifications we have considera bly simplified the generation of the repeat probe used to screen for a nomalous bands. This method will facilitate the molecular approach to other genetic disorders where expansions of repeat sequences could be involved.