SMALL-SCALE ISOLATION OF GENOMIC DNA FROM STREPTOMYCES-MYCELIA OR SPORES

We have developed a small-scale method for isolating high-quality chro mosomal DNA from Streptomyces species. The entire procedure may be car ried out in 2-mL microcentrifuge tubes in one day. It has been tested both quantitatively and qualitatively to ensure reliability and reprod ucibility. DNA yields from a variety of Streptomyces species ranged fr om 30 mu g DNA/50 mg cells to over 225 mu g DNA/50 mg cells. We used t he method to isolate DNA from cells grown in liquid culture, on solid media and from spore suspensions. DNA yields and quality were assessed by spectrophotometry, restriction endonuclease digestion and random-a mplified polymorphic DNA-PCR (RAPD-PCR) analysis. These confirmed that this procedure is an efficient method for isolating large amounts of high-quality DNA from a wide range of Streptomyces species.