EFFECT OF POLYDEXTROSE ON ABSORPTION OF TRIGLYCERIDE AND CHOLESTEROL IN MESENTERIC LYMPH-FISTULA RATS

In most experimental designs, the inhibitory effect of water-soluble d ietary fibers on lipid absorption is evaluated by the decrease in plas ma lipid concentration or the increase in fecal lipid output, The aim of this study was to evaluate the acute effect of a water-soluble poly saccharide, polydextrose, on lipid transport to the mesenteric lymph u sing lymph-fistula rats, The mesenteric lymph duct of rats was cannula ted, and an infusion tube was introduced into the duodenum, After reco very, a lipid emulsion containing radioactive triolein and cholesteryl oleate was infused into the duodenum for 8 hr. The tested group was i nfused with the lipid emulsion containing 5% or 10% polydextrose as di etary fiber, Samples from the lymph-fistula were collected, and the lu minal contents and mucosa were collected at the end of infusion. Lymph flow in the mesenteric lymph decreased in the polydextrose group afte r the infusion, The amounts of both triglyceride and cholesterol remai ning in the lumen were greater in the polydextrose group, due to decre ased transport of lipid into the lymph, These effects were dose depend ent in the 5% and 10% polydextrose groups, The of this study indicate that polydextrose retarded the transport of triolein and cholesterol i nto the mesenteric lymph.