Jt. Bergthorsson et al., CHROMOSOME IMBALANCE AT THE 3P14 REGION IN HUMAN BREAST-TUMORS - HIGH-FREQUENCY IN PATIENTS WITH INHERITED PREDISPOSITION DUE TO BRCA2, European journal of cancer, 34(1), 1998, pp. 142-147
Our previous studies have indicated that genetic aberrations in the 3p
14 region are more frequent in malignant tumours from hereditary breas
t cancer patients than sporadic breast cancers. The main purpose of th
is study was to test if BRCA2 susceptibility alleles contribute to imb
alance in the 3p14 region. We mapped allelic imbalance at 3p14 in tumo
urs from Icelandic sisters affected with breast cancer using a set of
10 microsatellite markers 1233-D3S1217-D3S1261-D3S1296-D3S1210-D3S1284
-cen). The patients were of known carrier status with respect to the 9
99del5 mutation in BRC42 which is the most common cause of hereditary
breast cancer in Iceland. Of 103 patients, 32 in the group were mutati
on carriers. A high degree of imbalance was observed in tumours from B
RCA2 mutation carriers, ranging from 44 to 88% for individual markers.
This was significantly higher than the percentage of imbalance in tum
ours from non-carriers, where the frequency ranged from 25 to 43%. In
both groups, we noted elevated 3p14 imbalance in patients with bilater
al disease. Allelic imbalance was most commonly observed near the mark
er D3S1210 (3p14.1-p12) and the FHIT gene (3p21.1-p14.2) for both grou
ps. We conclude that genomic aberrations in 3p14 are especially freque
nt in tumours with BRCA2 gene defects, and suggest that this is caused
by regional loss of chromosome stability rather than selection. (C) 1
998 Elsevier Science Ltd.