ANTIBACTERIAL STEROIDAL ALKALOIDS FROM SARCOCOCCA-SALIGNA

Two new pregnane-type steroidal alkaloids, saligcinnamide [(20S,2'E)-2 0-(N,N-dimethylamino)3 beta-(3'-phenyl-2'-propenyl-N-methylamido)] (1) and N-a-methyl epipachysamine-D[(20S)-20-(N,N-dimethylamino)-3 beta-( N-methylbenzamido)pregnane] (2), along with a known base, epipachysami ne D [(20S)-20-(N,N-dimethylamino)-3 beta-(benzamido)pregnane] (3), we re isolated from the EtOH extracts of the roots and stems of Sarcococc a saligna. The new bases exhibited antibacterial activity against seve ral human pathogenic bacteria. Two derivatives of 1, dihydrosaligcinna mide [(20S)-20-(N,N-dimethylamino)-3 beta-(3'-phenylpropionoyl-N-methy lamido)pregnane] (4) and dihydrosaligcinnamine [(20S)-20-(N,N-dimethyl amino)-3 beta-N-(3'-phenylpropyl-N- methylamino)pregnane] (5), and a d erivative of 1, N-a-methyl epipachysamine [(20S)-20-(N,N-dimethylamino )-3 beta-(N-benzyl, N-methylamino)pregnane] (6) were prepared and thei r antibacterial activity determined.