DESTRUXIN-A4 CHLOROHYDRIN, A NOVEL DESTRUXIN FROM FUNGUS OS-F68576 - ISOLATION, STRUCTURE DETERMINATION, AND BIOLOGICAL-ACTIVITY AS AN INDUCER OF ERYTHROPOIETIN

In the course of screening for small-molecule modulators of erythropoi etin gene expression, five destruxins were isolated from the fungal cu lture of OS-F68576. The structures were elucidated by extensive H-1 an d C-13 NMR spectroscopy and by hydrolytic modification. One compound ( destruxin-A4 chlorohydrin, 1) is a novel destruxin. All these compound s induced erythropoietin gene expression 5-fold at concentration of 0. 2-2 mu M.