TRANSDUCTION OF RECOMBINANT-HUMAN-ERYTHROPOIETIN RECEPTOR CDNA INTO DAUGHTER PROGENITORS DERIVED FROM SINGLE CD34(3-BLOOD CELLS CHANGES THEDIFFERENTIATION PROFILE OF DAUGHTER PROGENITORS() CORD)

In this study, we tested the capacity to change the differentiation pr ofile of progenitor cells by retroviral-mediated transduction of EpoR cDNA into one of the paired daughter cells derived from single CD34(3) CB cells, Our results show that for the non-viral-treated daughter c ells, the majority (99.6%) formed the same colony type. However, with cells transduced with viral vectors, 7.1% of the daughter cells transd uced with the EpoR cDNA formed either a burst forming unit-erythroid ( BFU-E) or a colony-forming unit-granulocyte, macrophage, erythroid, me gakaryocyte (CFU-GEMM) colony compared to the other daughter cell. tra nsduced with viral supernatant lacking EpoR cDNA, which formed either a colony-forming unit granulocyte-macrophage (CFU-GM) or a high prolif erative potential-colony forming cell (HPP-CFC) colony, Expression of the transduced EpoR cDNA was confirmed iu individual colonies by RT-PC R analysis, These results substantiate in a more rigorous fashion our previous results that it is possible to change the Epo-responsive diff erentiation profile of progenitor cells by transduction into these cel ls of an EpoR cDNA and this change was apparent only in daughter cells derived from single CD34(3+) kit(+) cells transduced with EpoR cDNA.