FINE-STRUCTURE AND ASSEMBLY IN-VITRO OF NUCLEAR LAMINA IN PLANT-CELLS

The fine structure of the nuclear lamina (NL) in sperm cells of Ginkgo biloba was visualised using high resolution low-voltage scanning elec tron microscopy (LVSEM). It was shown that the nuclear lamina was comp osed of 10 nm filaments which formed a fine network. Lamins were purif ied from cultured carrot suspension cells and assembled in vitro. Long 8-12 nm diameter filaments were seen and sometimes subfilaments could be distinguished. Western blot of filament preparations showed that t hese contained the 66 and 84 ku lamins. These data demonstrate that pl ant lamins are capable of assembling into filaments in vitro.