ROSMANOL CONTROLS EXPLANTS BROWNING OF HYPERICUM-CANARIENSIS L. DURING THE IN-VITRO ESTABLISHMENT OF SHOOTS

An efficient method for eradication of browning exudate was developed for Hypericum canariensis. For this purpose the effect of natural prod ucts on browning exudates were investigated in four types of culture m edia: Murashige and Skoog (MS, 1962); Gamborg's (B5, 1979); Woody Plan t Medium (WPM, Lloyd and McCown 1981) and modified Quoirin and Lepoivr e (QL.4) (Mederos 199L, Mederos et al. 1995) basal macroelements; thes e basal macroelements were supplemented with the microelement formula described by Murashige and Skoog (MS, 1962). During the establishment of shoots organogenesis potential was achieved in the Murashige and Sk oog(MS, 1962) and modified Quoirin and Lepoivre (QL. 4) (Mederos 1991, Mederos et al. 1995) media after browning exudates was eliminated by rosmanol treatments. Rosmanol is a powerful diterpenic antioxidant iso lated from Salvia canariensis L., a medicinal species endemic to the C anary Islands.