SIMULTANEOUS DETERMINATION OF PSEUDOEPHIDRINE HCL (PSE) AND TERFANIDINE (TER) FROM FORMULATIONS BY REVERSED-PHASE ION-PAIR HIGH-PERFORMANCELIQUID-CHROMATOGRAPHY (RP-HPLC)

A new simple, precise, rapid, and selective reversed-phase ion pair hi gh-performance liquid chromatography (RP-HPLC) method has been develop ed for the simultaneous determination of pseudoephidrine (PSE) and ter fanidine (TER) from tablets using 60:15:25 acetontrile:methanol:water (v/v) containing 2.9 g sodium lauryl sulfate/liter, pH adjusted to 3.1 using phosphoric acid as a mobile phase and C-18 Spherisorb ODS 2 (3 mu m, 5 cm x 4.6 mm i.d.) as stationary phase. Detection was carried o ut using a UV detector at 254 nm. A constant flow of 1.0 ml/min was ma intained throughout the analysis. Retention times for PSE and TER were 1.90 and 7.35 min, respectively. Linearity range and percentage recov eries for PSE and TER were 24-1200 and 12-600 mu g/ml, and 100.01 and 100.4%, respectively.