MEIOTIC PRODUCTS OF A KLINEFELTER 47,XXY MALE AS DETERMINED BY SPERM FLUORESCENCE IN-SITU HYBRIDIZATION ANALYSIS

The meiotic segregation of 24 spermatozoa obtained from a 47,XXY male is described. Three-colour fluorescence insitu hybridization with prob es for chromosomes X, Y and 18 was used. Five spermatozoa carried an X chromosome, seven carried a Y, six had an XY gonosomal complement, fi ve were missing the sex chromosome and one spermatozoon was presumably diploid with an XX/1818 complement. Our results support the hypothesi s that XXY cells are able to complete meiosis. In this patient, the pe rcentage of spermatozoa with an abnormal number of sex chromosomes inc reased from 1/6 (17%) among spermatozoa with normal morphology to 11/1 8 (61%) in spermatozoa with abnormal morphology.