SYNTHESIS AND LANGMUIR FILM FORMATION OF N-(EPSILON-MALEIMIDOCAPROYL)(DILINOLEOYLPHOSPHATIDYL) ETHANOLAMINE

imidocaproyl)(dilinoleoylphosphatidyl)ethanolamine (DLiPE-EMC) has bee n synthesized, representing a new polymerizable lipid with a linking g roup for covalent attachment of proteins for immunosensor applications . The linker lipid could be prepared by performing both the synthesis and separation steps under argon and avoiding heating. The product was characterized by 600 MHz H-1 NMR, mass, and UV-vis spectrometry. Mono molecular films were successfully prepared on a Langmuir trough, and t he characteristic surface pressure-area curves were measured on differ ent subphases, A condensation of the monolayer could be achieved by in troducing a micromolar concentration of uranyl ions in the subphase. S urface pressure-area isotherms of pure and mixed DLiPE/DLiPE-EMC monol ayers were also measured. UV irradiation experiments were performed to demonstrate the polymerizability of the mixed monolayers.