NONRADIOACTIVE SEQUENCE-TAGGED MICROSATELLITE SITE ANALYSES - A METHOD TRANSFERABLE TO THE TROPICS

Authors
LAGODA PJL DAMBIER D GRAPIN A BAURENS FC LANAUD C NOYER JL
Citation
Pjl. Lagoda et al., NONRADIOACTIVE SEQUENCE-TAGGED MICROSATELLITE SITE ANALYSES - A METHOD TRANSFERABLE TO THE TROPICS, Electrophoresis, 19(2), 1998, pp. 152-157
Citations number
29
Categorie Soggetti
Biochemical Research Methods","Chemistry Analytical
Journal title
Electrophoresis → ACNP
ISSN journal
01730835
Volume
19
Issue
2
Year of publication
1998
Pages
152 - 157
Database
ISI
SICI code
0173-0835(1998)19:2<152:NSMSA->2.0.ZU;2-7
Abstract
Utilization of existing isozyme analysis facilities to detect sequence -tagged microsatellite site (STMS) polymorphism or any simple sequence repeat (SSR) variation is described. Different parameters concerning the difficulties in transferring molecular techniques to less sophisti cated laboratory infrastructures (i.e. tropical outstations) are discu ssed (e.g. reproducibility, efficacy, precision). Nonradioactive STMS analysis is bound to foster collaborative research between ''biodivers ity'' and ''biotechnology'' centers.