ACTIVITY STAINING ON POLYACRYLAMIDE GELS OF TRYPSIN-INHIBITORS FROM LEAVES OF SWEET-POTATO (IPOMOEA-BATATAS L. LAM) VARIETIES

The failure of activity staining of trypsin inhibitors in crude leaf e xtracts of sweet potatoe varieties including Tainong 27, Tainong 34, a nd Tainong 57 on a 15% sodium dodecyl sulfate-polyacrylamide gel elect rophoresis (SDS-PAGE) gel was prevented by dipping the gels in solutio ns containing 10-40 mM hydrogen peroxide, 10 mM Tris buffer (pH 7.9) f or 30 min before staining.