TMIG-2DPAGE - A NEW CONCEPT OF 2-DIMENSIONAL GEL PROTEIN DATABASE FORRESEARCH ON AGING

Cellular proteins of a normal human diploid fibroblast line (TIG-3) at various stages of replicative aging were resolved by horizontal isoel ectric focusing on an immobilized pH gradient, followed by vertical so dium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). Spot proteins were visualized by silver staining and quantitated by im age processing. All corresponding spots were matched among two-dimensi onal eel images, and variation profiles in relative abundance of indiv idual proteins during in vitro aging were classified into five categor ies, i.e., (i) increase, (ii) decrease, (iii) increase followed by dec rease, (iv) decrease followed by increase, and (v) irregular or nonsig nificant variation. The new concept of the Tokyo Metropolitan Institut e of Gerontology two-dimensional gel protein database (TMIG-2DPAGE) wa s prepared from the above data to support research on cellular aging. The database was put on our World Wide Web home page at the URL of htt p://www.tmig.or.jp/2D/ to allow free access through the Internet. The individual protein data entries were linked to the standard spot prote in map of the two-dimensional gel image in order to be accessible by c licking the mouse on it.