RATES OF CELL-PROLIFERATION IN ADENOMATOUS, SUPPRESSED, AND NORMAL PARATHYROID TISSUE - IMPLICATIONS FOR PATHOGENESIS

In previous studies, the birth rate of new cells in parathyroid adenom as measured at the time of surgical excision was shown to be much too low to account for growth of the tumors from a single cell in the time available, but comparison with normal rates was not possible. We meas ured the prevalence of cells expressing the Ki-67 antigen, a cell cycl e marker, in 55 parathyroid adenomas using the MIB-1 antibody and micr owave antigen retrieval; in 22 cases, separate measurements were made in nonadenomatous tissue from the same glands. In 10 cases complete ma ps of the gland profile were reconstructed to study the distribution o f labeled cells. The proportion of Ki-67-positive cells, estimated by systematic random sampling, was used to calculate cell birth rate assu ming a duration of Ki-67 expression of 24 h; the results were compared to rates previously determined in normal parathyroid glands by the sa me method. The geometric mean cell birth rate was 9.97%/yr, about doub le the normal rate of 5.4%/yr, but less than a third of the cases had values above the normal range. The corresponding value in nonadenomato us tissue was 2.58%/yr, about half the normal rate. In 10 cases studie d in more detail, the cell birth rate was 12.3%/yr in the peripheral r egions and 6.2%/yr in the central regions, a value not significantly d ifferent from normal. The results in adenomas are in reasonable agreem ent with previous estimates of cell birth rate of 13.7%/yr using [H-3] thymidine labeling and 6.4%/yr using prevalence of the mitotic karyoty pe. The proportion of Ki-67-positive cells using unbiased sampling was about 50 times smaller than that in previous studies using selective sampling. Cell birth rates at the time of excision were about 20-25 ti mes lower than initial rates estimated from modeling tumor growth by t he Gompertz function. We conclude that 1) cell birth rate in parathyro id adenomas has fallen substantially during the growth of the tumors a nd is only modestly greater than normal; 2) the fall in cell birth rat e had been greater in the central and presumably older regions of the adenoma than in the peripheral and presumably younger regions; 3) nona denomatous tissue was suppressed with respect to its proliferative as well as its secretory function, presumably as a result of hypercalcemi a; and 4) the progressive fall in cell birth rate, despite the accumul ation of mutations that are supposed to increase cell birth rate, is m ost readily explained by the set-point hypothesis.