Spontaneous gizzard adenocarcinoma was found in a 15-year-old male Hum
boldt penguin (Spheniscus humboldti). The lesser curvature of the gizz
ard wall was extensively and markedly thickened with a hard white tumo
ur mass. Histopathologically, the tumour in the gizzard consisted of t
wo structures: small to large acini lined by cuboidal or flattened cel
ls with poor stroma in the mucosa, and undifferentiated tumour cells a
rranged singly or in cords with acinar structures separated by dense f
ibrous tissue in the muscular layer. Small white metastatic tumours of
the intestine and the pancreas had a similar histological appearance
to that in the muscular layer of the gizzard. From the location of tum
our in the gizzard and the stainability with combined alcian blue pH 2
.5-periodic acid Schiff, it was considered that this tumour might orig
inate from the lining cells of the tip of the gizzard mucosal folds.