HYDROLYTIC CLEAVAGE OF ADENOSINE-3'-MONOP HOSPHATE AND GUANOSINE-3'-MONOPHOSPHATE BY LANTHANIDES

The hydrolytic cleavage of adenosine-3'-monophosphate (3'-AMP) and gua nosine-3'-monophosphate (3'-GMP) by lanthanides was investigated by NM R, HPLC and the method of measuring the liberated inorganic phosphate. The results show that lanthanides have specificity for hydrolyzing 3' -mononucleotides. 3'-AMP and 3'-GMP were converted to Adenosine (A), p hosphate and Guanosine (G),phosphate respectively at pH 9, 37 degrees C. The efficiency of cleavage was greater than that of 5'-mononucleoti des. The mechanism of hydrolytic of cleavage was discussed in detail.