PREPARATION OF PH-SENSITIVE PHASE-SEPARAT ING POLYMERS AND ITS APPLICATION IN IMMUNOASSAY

Authors
ZHOU P DENG YZ ZENG YE
Citation
P. Zhou et al., PREPARATION OF PH-SENSITIVE PHASE-SEPARAT ING POLYMERS AND ITS APPLICATION IN IMMUNOASSAY, Gaodeng xuexiao huaxue xuebao, 19(2), 1998, pp. 198-200
Citations number
4
Categorie Soggetti
Chemistry
Journal title
Gaodeng xuexiao huaxue xuebao → ACNP
ISSN journal
02510790
Volume
19
Issue
2
Year of publication
1998
Pages
198 - 200
Database
ISI
SICI code
0251-0790(1998)19:2<198:POPPIP>2.0.ZU;2-N
Abstract
The methylmethacrylate-acrylic acid-maleic anhydrate copolymer(MAM) an d methacrylic acid-acrylamide-N-acryloxysuccinimide copolymer(MAN) wer e prepared. These copolymers were conjugated with monoclonal antibody separately and utilized in a novel separation method for enzyme immuno assay. The polymer precipitated out of water below a critical pH, enab ling the polymer-bound immune complex to be separated from the solutio n. However, the MAM-antibody can show phase separation properties in u rine, but can not precipitate in serum. The MAN-antibody showed the in soluble form below PH 3.5 and soluble form above pH 4.0. It has been d emonstrated to be applicable to determination of antigen by determinin g HBsAg in serum. Forty-five samples were assayed, and there was a goo d correlation between the results by this method and ELISA.