ESTIMATION OF TYPE-SPECIFIC ANTISTREPTOCOCCAL M-PROTEIN ANTIBODIES WITH BIOTINYLATED PEPTIDES IN HUMAN SERA

Authors
KUHNEMUND O SCHUMACHER F CASARETTO M HOLM SE LUTTICKEN R
Citation
O. Kuhnemund et al., ESTIMATION OF TYPE-SPECIFIC ANTISTREPTOCOCCAL M-PROTEIN ANTIBODIES WITH BIOTINYLATED PEPTIDES IN HUMAN SERA, APMIS. Acta pathologica, microbiologica et immunologica Scandinavica, 106(2), 1998, pp. 288-292
Citations number
19
Categorie Soggetti
Pathology,Microbiology,Immunology
Journal title
APMIS. Acta pathologica, microbiologica et immunologica Scandinavica → ACNP
ISSN journal
09034641
Volume
106
Issue
2
Year of publication
1998
Pages
288 - 292
Database
ISI
SICI code
0903-4641(1998)106:2<288:EOTAMA>2.0.ZU;2-Z
Abstract
The design of a novel enzyme-linked immunosorbent assay for the estima tion of antibodies directed against the N-terminus of the M-protein of Streptococcus pyogenes is described. The ELISA employs biotinylated p eptide antigens of the types 1, 4, 12 and 19 immobilized by (strept-)a vidin on the surface of polystyrene microtiter wells. In rabbit hyperi mmune sera and in human serum samples, antibodies against the correspo nding serotype could be detected with high sensitivity and specificity .