O. Kuhnemund et al., ESTIMATION OF TYPE-SPECIFIC ANTISTREPTOCOCCAL M-PROTEIN ANTIBODIES WITH BIOTINYLATED PEPTIDES IN HUMAN SERA, APMIS. Acta pathologica, microbiologica et immunologica Scandinavica, 106(2), 1998, pp. 288-292
The design of a novel enzyme-linked immunosorbent assay for the estima
tion of antibodies directed against the N-terminus of the M-protein of
Streptococcus pyogenes is described. The ELISA employs biotinylated p
eptide antigens of the types 1, 4, 12 and 19 immobilized by (strept-)a
vidin on the surface of polystyrene microtiter wells. In rabbit hyperi
mmune sera and in human serum samples, antibodies against the correspo
nding serotype could be detected with high sensitivity and specificity
.