ROLE OF CUTICLE-DEGRADING PROTEASES IN THE VIRULENCE OF METARHIZIUM SPP. FOR THE DESERT LOCUST, SCHISTOCERCA-GREGARIA

The production of the subtilisin proteinase, PR1, and the trypsin-like enzyme, PR2, in liquid cultures of 19 isolates of Metarhizium spp. wa s investigated. PR1 activity was generally observed 72 h after inocula tion whilst PR2 activity was detected in most strains 48 h after inocu lation. PR1 was partially purified from each isolate of Metarhizium sp p. The ability of this enzyme from each isolate to degrade different t ypes of insect cuticle was investigated. The cuticle types used were M anduca sexta pupal cuticle, adult desert locust (Schistocerca gregaria ) wing cuticle, abdominal cuticle, and pharate adult locust abdominal cuticle. These cuticle types differ in their protein composition and d egree of sclerotization. PR1 hydrolyzed the locust cuticle to differen t degrees. The data suggest a hierarchy for the susceptibility to hydr olysis in the order pharate adult abdominal > adult abdominal > wing. There was no significant correlation between the ability of the enzyme s to degrade any of the cuticle types and median lethal time of the is olates against the desert locust. However, a correlation was found bet ween the ability of enzymes to hydrolyze different locust cuticles. Su ch correlations were not apparent to the same extent between M. sexta cuticle and the locust cuticles. These results are discussed in terms of a possible contribution of PR1 to host specificity. (C) 1998 Academ ic Press.