STEM-CELL FACTOR IN HUMAN SEMINAL PLASMA AS A MARKER FOR SPERMATOGENESIS

Objectives. To measure the level of stem cell factor (SCF) in human se minal plasma to determine whether SCF may be useful in evaluating the ability to produce sperm and search the role of SCF in the testes. Met hods, We measured the level of SCF in seminal plasma obtained from 108 males, including idiopathic azoospermia due to germ cell aplasia (n = 10), oligospermia (n = 50), asthenospermia (n = 31), and normospermia (n = 17). The expression of SCF messenger ribonucleic acid in the hum an testis was evaluated by reverse transcriptase polymerase chain reac tion (RT-PCR). The correlation between its level and clinical findings was also evaluated. Results. RT-PCR showed a larger form that encoded the soluble protein and a smaller form that encoded the membrane-asso ciated form of SCF in the human testis. The similar ratio of the large r form to the smaller one was observed both in the testis of normal an d oligospermic men. The level of SCF is significantly correlated with the sperm count (r = 0.214; P < 0.05). Conclusions. The level of SCF i n seminal plasma appeared to predict the ability to produce sperm. Thu s, this factor may play an important role in spermatogenesis. (C) 1998 , Elsevier Science Inc. All rights reserved.