A GENETIC-LINKAGE MAP OF RYE (SECALE-CEREALE L.)

A genetic linkage map of rye composed of 91 loci (88 RFLP, two morphol ogical and one isozyme markers) has been developed using two reciproca l crosses. The RFLP loci covering all seven chromosomes were detected by a selection of rye, wheat, barley and oat cDNA and genomic DNA prob es. The level of polymorphism was dependent on the source of the clone s, with a ranking of rye > wheat > barley > oat. Distorted segregation s were detected in linkage groups of chromosomes 1R, 4R, 5R and 7R. Wh en the recombination of the two reciprocal crosses was compared, no sy stematic increase or decrease in one or the other direction was observ ed suggesting that a combination of populations of reciprocal crosses is possible.