Plantago major plants from several Scottish and Dutch locations were s
urveyed for their genetic variation using PCR markers, namely RAPD ana
lysis, anchored inter-SSR PCR, and chloroplast PCR followed by RFLP an
alysis. The RAPD and inter-SSR markers showed a differentiation betwee
n the two subspecies of P. major. These results are discussed in relat
ion to earlier results using allozyme electrophoresis, DNA fingerprint
ing, and chloroplast RFLP analysis.