ANALYSIS OF TWEEN-80 AS AN ESTERASE LIPASE SUBSTRATE FOR LIPOLYTIC-ACTIVITY ASSAY/

Authors
PLOU FJ FERRER M NUERO OM CALVO MV ALCALDE M REYES F BALLESTEROS A
Citation
Fj. Plou et al., ANALYSIS OF TWEEN-80 AS AN ESTERASE LIPASE SUBSTRATE FOR LIPOLYTIC-ACTIVITY ASSAY/, Biotechnology techniques, 12(3), 1998, pp. 183-186
Citations number
11
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
3
Year of publication
1998
Pages
183 - 186
Database
ISI
SICI code
0951-208X(1998)12:3<183:AOTAAE>2.0.ZU;2-A
Abstract
The Tween 80 assay to detect lipolytic activities in agar media was ev aluated. A spectrophotometric assay for Tween 80 hydrolysis was establ ished. The specific activities with Tween 80, as well as with some con ventional lipase-type and esterase-type substrates, were measured usin g several lipases and esterases. The activity with Tween 80 was simila r to that obtained with p-nitrophenyl butyrate; the enzyme activities with both substrates were between the esterase and lipase categories.