Alginate beads and those immobilised with Chlorella vulgaris were suit
able for repeated adsorption/desorption cycling for copper under batch
conditions. Consistently high copper removal (>95%) and recovery (100
% using 0.1 M HCl) were achieved in five consecutive cycles. Majority
copper was adsorbed by alginate matrix, not the cells. Freely suspende
d cells were difficult to handle in repeated cycling, and given incons
istent copper removal and recovery. Heat killed cells performed the sa
me as initially live cells after the first cycle.