OXIDATION OF 1-HYDROXYBENZOTRIAZOLE BY LACCASE AND LIGNIN PEROXIDASE

Authors
ANDER P MESSNER K
Citation
P. Ander et K. Messner, OXIDATION OF 1-HYDROXYBENZOTRIAZOLE BY LACCASE AND LIGNIN PEROXIDASE, Biotechnology techniques, 12(3), 1998, pp. 191-195
Citations number
13
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
3
Year of publication
1998
Pages
191 - 195
Database
ISI
SICI code
0951-208X(1998)12:3<191:OO1BLA>2.0.ZU;2-L
Abstract
A method to measure laccase and lignin peroxidase (LiP) activity at 40 8 nm (402-410 nm) using 1-hydroxybenzotriazole (HBT) was developed. Th e assay can be performed either as a kinetic measurement or as a stopp ed reaction using 5 mM Na-azide which improves the spectrum. Only whit e-rot fungal laccases and LiP were found to oxidize HBT to give should ers or peaks at 402-410 nm. Phanerochaete and Phlebia manganese peroxi dases did not give absorbance increase at 402-410 nm.