Mb. Medina et Pm. Fratamico, BINDING INTERACTIONS OF COLLAGEN-I, LAMININ AND FIBRONECTIN WITH IMMOBILIZED ESCHERICHIA-COLI O157-H7 USING A SURFACE-PLASMON RESONANCE BIOSENSOR, Biotechnology techniques, 12(3), 1998, pp. 235-240
Citations number
28
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Real-time interactions of collagen I, fibronectin, laminin, hyaluronic
acid and chondroitin sulfate with immobilized Escherichia coli O157:H
7 cells were studied with a surface plasmon resonance biosensor. Resul
ts showed that collagen I and laminin bound to the E. coli surface but
fibronectin had very low binding while hyaluronic acid and chondroiti
n sulfate had no detectable interaction. Calcium ion inhibited laminin
binding but enhanced collagen I binding. This research provides a mod
el system to study the interactions of bacterial cells with extracellu
lar matrix components.