MICROSCALE PURIFICATION OF BETA-GALACTOSIDASE FROM KLUYVEROMYCES-LACTIS REVEALS THAT DIMERIC AND TETRAMERIC FORMS ARE ACTIVE

Authors
BECERRA M CERDAN E SISO MIG
Citation
M. Becerra et al., MICROSCALE PURIFICATION OF BETA-GALACTOSIDASE FROM KLUYVEROMYCES-LACTIS REVEALS THAT DIMERIC AND TETRAMERIC FORMS ARE ACTIVE, Biotechnology techniques, 12(3), 1998, pp. 253-256
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
3
Year of publication
1998
Pages
253 - 256
Database
ISI
SICI code
0951-208X(1998)12:3<253:MPOBFK>2.0.ZU;2-J
Abstract
beta-Galactosidase was purified from Kluyveromyces lactis. Polyclonal antibodies raised against it cross-reacted against the beta-galactosid ase of Kluyveromyces fragilis but not against beta-galactosidases of b acterial, fungal or animal origin. The enzyme is composed of 124 kDa m onomers and is active in both dimeric and tetrameric form.