DDGGE, A SIMPLIFIED DENATURING GRADIENT GEL-ELECTROPHORESIS METHOD FOR THE DETECTION OF SEQUENCE VARIATIONS IN PCR FRAGMENTS

Authors
LOGEMANN S JETTEN MSM
Citation
S. Logemann et Msm. Jetten, DDGGE, A SIMPLIFIED DENATURING GRADIENT GEL-ELECTROPHORESIS METHOD FOR THE DETECTION OF SEQUENCE VARIATIONS IN PCR FRAGMENTS, Biotechnology techniques, 12(3), 1998, pp. 263-265
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
3
Year of publication
1998
Pages
263 - 265
Database
ISI
SICI code
0951-208X(1998)12:3<263:DASDGG>2.0.ZU;2-Y
Abstract
The need for rapid analysis of sequence variations in PCR fragments of the same length is increasing in medical diagnostics and environmenta l studies. Therefore a modified denaturing gradient gel electrophoresi s (DGGE) method was developed in which mixed PCR fragments of 1,500 bp could be analysed on a conventional DNA sequencing gel apparatus. In addition, PCR primers without long GC-clamps could be used to amplify the target genes.