COMPARATIVE-ANALYSIS OF INTRACELLULAR PROTEASES IN SPORULATED BACILLUS-THURINGIENSIS STRAINS

Citation
St. Reddy et al., COMPARATIVE-ANALYSIS OF INTRACELLULAR PROTEASES IN SPORULATED BACILLUS-THURINGIENSIS STRAINS, Biotechnology letters, 20(3), 1998, pp. 279-281
Citations number
19
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
ISSN journal
01415492
Volume
20
Issue
3
Year of publication
1998
Pages
279 - 281
Database
ISI
SICI code
0141-5492(1998)20:3<279:COIPIS>2.0.ZU;2-X
Abstract
Intracellular proteases in three strains of sporulated Bacillus thurin giensis were identified following fractionation with ammonium sulfate. The major proteolytic activity in subsp. tenebrionis and subsp. kurst aki belonged to the metalloprotease class while in subsp. israelensis, 50 % activity was contributed by serine proteases. Protease activitie s in subsp. kurstaki (92 kDa, 78 kDa and 69 kDa) and subsp. israelensi s (90 kDa, 75 kDa, 60 kDa and 30 kDa) were detected by gelatin SDS-PAG E.