OLIGOSACCHARIDE SYNTHESIS BY INVERTASE IN ORGANIC MEDIA CONTAINING SDS

Authors
BIELECKI S SOMIARI RI
Citation
S. Bielecki et Ri. Somiari, OLIGOSACCHARIDE SYNTHESIS BY INVERTASE IN ORGANIC MEDIA CONTAINING SDS, Biotechnology letters, 20(3), 1998, pp. 287-290
Citations number
13
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
20
Issue
3
Year of publication
1998
Pages
287 - 290
Database
ISI
SICI code
0141-5492(1998)20:3<287:OSBIIO>2.0.ZU;2-B
Abstract
Oligosaccharide synthesis by invertase increased from 3.1 to 12.1% (w/ w) in medium containing 1.7 M sucrose and 60% (v/v) butyl acetate when 90 mM SDS was added. The main oligosaccharide synthesised has a MW of 504 Da and is made up of 2 fructose units and 1 glucose. Reversed mic elles were not formed with SDS addition, but invertase activity was en hanced by over 30% in all the organic two-phase systems studied.