PRODUCTION OF A PERILLYL ALCOHOL-DEHYDROGENASE BY SITE-DIRECTED MUTAGENESIS OF A BENZYL ALCOHOL-DEHYDROGENASE

Authors
GILLOOLY DJ FEWSON CA
Citation
Dj. Gillooly et Ca. Fewson, PRODUCTION OF A PERILLYL ALCOHOL-DEHYDROGENASE BY SITE-DIRECTED MUTAGENESIS OF A BENZYL ALCOHOL-DEHYDROGENASE, Biotechnology letters, 20(3), 1998, pp. 325-327
Citations number
23
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
20
Issue
3
Year of publication
1998
Pages
325 - 327
Database
ISI
SICI code
0141-5492(1998)20:3<325:POAPAB>2.0.ZU;2-V
Abstract
Benzyl alcohol dehydrogenase from Acinetobacter calcoaceticus oxidises a wide range of aromatic and other cyclic alcohols and it has high sp ecificity constants for these substrates, but it does not oxidise shor t- or long-chain aliphatic alcohols. Mutation of an active-site argini ne to a histidine can switch the substrate specificity of the enzyme s o that it has a very much greater preference for perillyl alcohol than for benzyl alcohol.