INHIBITION OF MYCELIAL GROWTH OF PHAEOLUS-SCHWEINITZII BY HYDROLYSATES DIFFUSED FROM CULTURED CONIFER MEGAGAMETOPHYTES

The megagametophytes of European black pine (Pinus nigra ARN.), Mugo p ine (Pinus mugo TURRA), Scots pine (Pinus sylvestris L.) and Norway sp ruce (Picea, abies (L.) KARST.) were precultured on modified MS (MURAS HIGE & SKOOG, 1962) agar medium. After their removing from the medium the tester basidiomycete Phaeolus schweinitzii was inoculated. Very st rong inhibition of mycelial growth by substances diffused from pines m egagametophytes was found. The hydrolysates from spruce megagametophyt es appeared to be weaker inhibitors of mycelial growth. Proteins diffu sed from pre-cultured megagametophytes were analysed by SDS-PAGE.