GAVAGE ADMINISTRATION OF THE FUNGAL TOXIN FUMONISIN B-1 TO FEMALE SPRAGUE-DAWLEY RATS

Citation
Gs. Bondy et al., GAVAGE ADMINISTRATION OF THE FUNGAL TOXIN FUMONISIN B-1 TO FEMALE SPRAGUE-DAWLEY RATS, Journal of toxicology and environmental health. Part A, 53(2), 1998, pp. 135-151
Citations number
28
Categorie Soggetti
Toxicology,"Environmental Sciences","Public, Environmental & Occupation Heath
ISSN journal
15287394
Volume
53
Issue
2
Year of publication
1998
Pages
135 - 151
Database
ISI
SICI code
1528-7394(1998)53:2<135:GAOTFT>2.0.ZU;2-Q
Abstract
The fungal toxin fumonisin B-1 (FB1) is a contaminant of corn-based fo ods and feeds produced by members of the genus Fusarium. Fumonisin B-1 toxicity was examined using gavage administration of purified toxin t o female Sprague-Dawley rats. For 11 consecutive days each rat receive d a single dose of FB, at the following concentrations: control (salin e), 1, 5, 15, 35, or 75 mg FB1/kg body weight/d. Significantly depress ed body weight and food consumption occurred at 35 and 75 mg FB1/kg/d. By the end of the dosing period there were no significant changes in food consumption. Kidneys and bone marrow were most sensitive to FB1 e xposure. Changes in renal morphology were observed from 5 to 75 mg FB1 /kg/d, accompanied by transient changes in urine osmolality and urine enzyme levels. increased cellular vacuolation was the primary change a ssociated with bone-marrow toxicity, starting at doses of 5 mg FB1/kg/ d. Hepatotoxicity was indicated by reduced liver weight, elevated seru m alanine amonitransferase (ALT), and mild histopathological changes o ccurring at doses of 15 mg FB1/kg/d and higher. Increased cytoplasmic vacuolation of adrenal cortex cells occurred in rats treated with 15 m g FB1/kg/d and higher, indicating that the adrenals are also potential targets of FB1. Elevated serum cholesterol, which is a consistent res ponse to FB1, was observed at 5 mg FB1/kg/d and higher. Based on respo nses in this study, gavage is an appropriate substitute for longer fee ding studies. Compared to previous work with male rats, gender-related differences in FB1 responses lacked consistency but indicated that ma les may be marginally more sensitive than female Sprague-Dawley rats.