A. Gorskabrylass et al., DISTRIBUTION OF LOOSELY-BOUND CALCIUM IN THE VEGETATIVE AND GENERATIVE CELLS OF THE POLLEN GRAINS IN CHLOROPHYTUM-ELATUM, Biologia plantarum, 40(2), 1998, pp. 169-181
We used chlorotetracycline fluorescence, alizarin staining and potassi
um pyroantimonate methods, as well as X-ray microanalysis, to demonstr
ate the differential localization of Ca2+ during pollen maturation. Le
vel of loosely-bound Ca2+ ions was higher in the generative cell than
in the vegetative cell of the mature pollen grain which is one of the
symptoms of metabolic differentiation of the two sister cells.