The aim of the present study was to determine the intra- and interobse
rver variation during measurement of muscle hardness with a new instru
ment, a so-called hardness meter. In addition, we investigated the fac
tors which may influence the recording of muscle hardness and whether
the hardness differs within the same muscle. Hardness was measured at
a standardized Feint on the trapezius muscle in 20 volunteers by 2 obs
ervers in random order and again by 1 of the observers after 30 min. M
uscle hardness was then measured at 3 different locations on the trape
zius muscle. Ln addition, the muscle hardness at the standard point wa
s measured in 10 volunteers by both observers at 3 different speeds of
the applied pressure. The intraobserver variation was 10% and the int
erobserver variation was 12% for recording of muscle hardness. There w
as no significant difference in hardness values from time to time with
in the same observer (p=0.12), while there was a significant differenc
e in hardness measurements between observers (p=0.007). Muscle hardnes
s differed significantly among the 3 anatomical locations (p=0.03). Fo
r both observers, there was a significant difference among hardness re
cordings obtained at 3 different speeds (p=0.01 and p=0.0001). In conc
lusion we have shown that the hardness meter can measure muscle hardne
ss reliably if the same observer is used throughout a study. The speed
factor was a major source of variability In addition, we have demonst
rated that muscle hardness differs within the same muscle. We suggest
that the muscle hardness meter will be an important tool in future res
earch of the mechanisms leading to myofascial pain.