APPLICATION OF A METHOD INCORPORATING TREATMENT WITH CHLORAMINE-T FORTHE SELECTIVE ISOLATION OF STREPTOSPORANGIACEAE FROM SOIL

A simple isolation method for rare actinomycetes belonging to the fami ly Streptosporangiaceae was developed, in which a water suspension of air-dried soil was first treated with chloramine-T (1.0%), a chlorine- releasing biocide, diluted in water, and subsequently cultured on plat es of humic acid-vitamin medium. The chlorination treatment significan tly inhibited the growth of non-filamentous bacteria and undesirable a ctinomycete contaminants on the plates, thereby facilitating the selec tive isolation of bacteria from the Herbidospora, Microbispora, Microt etraspora and Streptosporangium genera.