QUANTITATIVE RELATIONSHIP BETWEEN RETENTION IN HPLC AND CE VIA BINDING CONSTANTS AND SELECTOR CONCENTRATIONS

The use of capillary electrophoresis (CE) far the rapid analysis of ch iral compounds is gaining wide acceptance by industry. Highperformance liquid chromatography (HPLC;) is, however, still the preferred choice for chiral separations due to its robustness, reproducibility and det ection limits but is often limited by method development time and long analysis times. Outlined in this article is a systematic approach for the determination of isocratic HPLC conditions using CE as a rapid sc outing method where the separation occurs via the same specific bindin g process in each method, e.g. an inclusion process. A quantitative li nk between retention factors (k') in CE and HPLC is illustrated via th e binding constants (K) and the concentrations of selector used in eac h system. Good agreement is shown between predicted and experimental v alues, showing how powerful this systematic method development approac h can be. (C) 1998 Published by Elsevier Science B.V.