SIMULTANEOUS ANALYSIS OF BOVINE GROWTH-HORMONE AND PROLACTIN ALLELES BY MULTIPLEX PCR AND RFLP

A simplified method of simultaneous genotyping of bovine growth hormon e (bGH) and prolactin (bPRL) has been developed. The method is based o n simultaneous amplification of fragments of the two genes by multiple x PCR, and subsequent digestion of the products by two restriction enz ymes (Alu I and Rsa I). Digestion with Alu I distinguished growth horm one L and V alleles and did not cut within the prolactin fragment, whi le digestion with Rsa I allowed the differentiation between the A and B alleles of bovine prolactin. All combinations of the known alleles c ould be detected by electrophoretic separation on the same agarose gel . Frequency of the allele L of the bovine growth hormone gene in Slova k Pied, Slovak Pinzgauer and Holstein was 0.55, 0.55 and 0.72, respect ively. Frequency of the allele A of the bovine prolactin gene in the s ame breeds was 0.87, 0.68 and 0.95, respectively.