THE INOSITOL 1,4,5-TRISPHOSPHATE-GENERATING AGONIST ATP ENHANCES DNA CLEAVAGE INDUCED BY TERT-BUTYLHYDROPEROXIDE

Citation
E. Clementi et al., THE INOSITOL 1,4,5-TRISPHOSPHATE-GENERATING AGONIST ATP ENHANCES DNA CLEAVAGE INDUCED BY TERT-BUTYLHYDROPEROXIDE, Experimental cell research, 239(1), 1998, pp. 175-178
Citations number
21
Categorie Soggetti
Cell Biology
Journal title
ISSN journal
00144827
Volume
239
Issue
1
Year of publication
1998
Pages
175 - 178
Database
ISI
SICI code
0014-4827(1998)239:1<175:TI1AAE>2.0.ZU;2-7
Abstract
In this paper we present experimental evidence indicating that DNA cle avage induced by tert-butylhydroperoxide in U937 cells can be enhanced via ATP-mediated activation of membrane receptors coupled with hydrol ysis of phosphatidylinositol 4,5-bisphosphate. The mechanism whereby A TP exerts this effect involves release of Ca2+ from the inositol 1,4,5 -trisphosphate (IP3)-sensitive stores, further release of the cation f rom the ryanodine receptor, mitochondrial clearance of the fraction of Ca2+ derived from the ryanodine receptor, and Ca2+-dependent mitochon drial formation of DNA-damaging species. IP3-generating agonists must therefore be considered as potential modulators of the genotoxic effec ts of tert-butylhydroperoxide. (C) 1998 Academic Press.