N. Cunado et Jl. Santos, A METHOD FOR FLUORESCENCE IN-SITU HYBRIDIZATION AGAINST SYNAPTONEMAL COMPLEX-ASSOCIATED CHROMATIN OF PLANT MEIOCYTES, Experimental cell research, 239(1), 1998, pp. 179-182
An improved method of preparing two-dimensional surface spreads of pla
nt synaptonemal complexes (SCs) associated with fluorencent in situ hy
bridization is described. This technique produces clear preparations o
f SCs and, in addition, consistently reveals the organization and loca
tion of different repetitive DNA sequences in plant meiotic prophase c
hromosomes. (C) 1998 Academic Press.