A NEW METHOD FOR GMA SECTIONS - IMMUNOFLUORESCENCE WITH THE ELF-PRECIPITATE COMBINED WITH A CLASSICAL HEMATOXYLIN EOSIN-PHLOXIN STAIN/

Rat pancreas, embedded in 2-hydroxyethyl methacrylate (GMA), was used to demonstrate glucagon by immunofluorescence in combination with a he matoxylin/eosin-phloxin (H/E-P) stain. This method for GMA embedded ti ssue enables a good differentiation with both light and fluorescence m icroscopy. The enzyme-labeled-fluorescence (ELF) precipitate marked im munoreaction can be visualized with UV excitation, whereas the H/E-P s tain can be observed in both bright field and with blue or green excit ation. The ELF-signal together with the surrounding tissue structure c an be visualized with double bandpass filters that enable UV together with blue or green excitation. Alternatively a similar effect can be a chieved photographically by double exposure.