The aim of this paper is to rehabilitate informative potential of usin
g deuterium as a natural tracer in geofluids investigations and to ill
ustrate how the deuterium concentration from geological fluid links th
e micro-scale effects to the macro-scale processes. Research on the de
uterium concentration of crude oils formerly has highlighted the role
of deuterium as a natural tracer during diagenesis. We discuss how the
deuterium abundance in oil and water from sedimentary basins provides
information on dynamics of hydrocarbons reservoirs. We can obtain qua
litative and quantitative information about the dynamics of fluids fro
m deposit by deuterium concentration monitoring from geofluids (gas-oi
l-water).