MONOFILAMENT INTRALUMINAL MIDDLE CEREBRAL-ARTERY OCCLUSION IN THE MOUSE

Citation
Wm. Clark et al., MONOFILAMENT INTRALUMINAL MIDDLE CEREBRAL-ARTERY OCCLUSION IN THE MOUSE, Neurological research, 19(6), 1997, pp. 641-648
Citations number
26
Categorie Soggetti
Neurosciences,"Clinical Neurology
Journal title
ISSN journal
01616412
Volume
19
Issue
6
Year of publication
1997
Pages
641 - 648
Database
ISI
SICI code
0161-6412(1997)19:6<641:MIMCOI>2.0.ZU;2-F
Abstract
The rat middle cerebral artery (MCA) occlusion model with an intralumi nal filament is well characterized with a two hour period of occlusion in widespread use. The recent availability of transgenic animals has led to an interest in adapting the MCA model in the mouse. To date the model has not been well characterized in the mouse. We performed the present study to compare different durations of MCA occlusion and to v alidate new functional assessments in this model. The MCA occlusion mo del (5-0 filament) was used. Swiss-Webster mice, 24-44 g, were randoml y assigned to four groups: one hour of occlusion; two hours of occlusi on; three hours of occlusion; or permanent occlusion. At 48 hours post -ischemia, the animals were rated on three neurologic function scales, and then the brains were removed for lesion size determination. Overa ll, there was a significant difference in lesion volume (p < 0.001) be tween the groups. In the permanent group of mice, the average lesion v olume was 78.41 +/- 17.47 mm (n = 12); two and three hours of ischemia produced 51.29 +/- 29.82 mm(3) (n = 11) and 54.85 mm(3) (n = 13), res pectively, significantly different than the one hour group 14.84 +/- 3 1.34 mm(3) (n = 11). All three functional scoring systems found signif icant overall differences between the four groups with our detailed Ge neral and Focal scores producing more robust between group treatment d ifferences and showing correlation coefficients of r = 0.766 and r = 0 .788, respectively to infarct volume. The MCA filament occlusion model can be successfully adapted in the mouse with either two or three hou r occlusions producing reliable infarcts. New functional scoring syste ms unique to the mouse appear to add additional information.